stromal cell line hs  (ATCC)

Journal: Communications Biology

Article Title: A human pathophysiological 3D-bone marrow model reveals immune and stromal cell heterogeneity

doi: 10.1038/s42003-025-09433-6

Figure Lengend Snippet: a Schematic diagram of the 2D polarization protocol using PMA and cytokines. b Phagocytosis assay performed on THP-1 cells following 2D polarization, n = 3. c , d IL-1β, n = 12 ( c ) and TNF-α, n = 9 ( d ) levels measured by ELISA in supernatants from 2D-polarized THP-1 cells. e Schematic diagram of the 3D experimental setup. In contrast to the 2D condition (left, same as in a ), THP-1 cells were added on day 21 to the 3D-BOM model (right), composed of self-assembled mesenchymal and endothelial cells. No treatment was applied (NT). 3D-BOM models were dissociated on day 28 for flow cytometry analysis. f–k Percentage of CD14⁺ ( f ), CD11b⁺ ( g ), CD36⁺ ( h ), CD80⁺ ( i ), TLR4⁺ ( j ), and CD86⁺ ( k ) cells in 2D (n = 9) vs. 3D cultures (n = 3-6) using the gating strategy illustrated in Supplementary Fig. . NT: non-treated control; No: PMA-treated, no cytokines added. Hatched bar indicates PMA-treated cells. Data are presented as mean ± SEM; one-way ANOVA, followed by Tukey’s post hoc test. Each dot represents an independent experiment.

Article Snippet: The HS27-A human bone marrow-derived mesenchymal stromal cell line (ATCC, CRL-2496), Kasumi-1 (ATCC, CRL-2724) and THP-1 (ATCC, TIB-202) were cultured in RPMI1640 medium (Gibco, 71870-010) supplemented with 10% FBS and 1% P/S.

Techniques: Phagocytosis Assay, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Control

Journal: Communications Biology

Article Title: A human pathophysiological 3D-bone marrow model reveals immune and stromal cell heterogeneity

doi: 10.1038/s42003-025-09433-6

Figure Lengend Snippet: a Schematic diagram of the 2-step experimental procedure used to obtain a 3D-BOM model, using primary MSC. On day 21, primary samples of hematopoietic progenitors were added. The 3D models were processed on day 28 and used for single cell analysis. b UMAP visualisation of mesenchymal cluster identified in Figure . c Dot-plot of selected marker genes differentially expressed across stromal populations in the 3D-BOM model. d Pseudotime analysis (Slingshot) of cellular differentiation from MSC to chondrocyte progenitors (lineage 1) or osteoblasts (lineage 2). e CytoTRACE stemness scoring across mesenchymal population. f UMAP visualisation of endothelial clusters identified in Figure . g Endothelial cell types signature scores across endothelial cell sub-clusters (U-cell Scoring, reference genes lists based on Boueya et al. ). h Violin plots of gene expression levels in endothelial cells sub-clusters (EC). KITLG: KIT ligand, ENG: endoglin, COL1A1: collagen type I alpha 1 chain, COL1A2: collagen Type 1 Alpha 2 chain. g , h t-test comparisons.

Article Snippet: The HS27-A human bone marrow-derived mesenchymal stromal cell line (ATCC, CRL-2496), Kasumi-1 (ATCC, CRL-2724) and THP-1 (ATCC, TIB-202) were cultured in RPMI1640 medium (Gibco, 71870-010) supplemented with 10% FBS and 1% P/S.

Techniques: Single-cell Analysis, Marker, Cell Differentiation, Gene Expression